H7772

Higromicina B

lyophilized powder

• Sinônimo(s): Hygrovetine
• Fórmula empírica (Notação de Hill): C₂₀H₃₇N₃O₁₃
• Número CAS: 31282-04-9
• Peso molecular: 527.52
• Número CE: 250-545-5
• Número MDL: MFCD06795479
• Código UNSPSC: 51102829
• ID de substância PubChem: 24895807
• NACRES: NA.85

Propriedades

• fonte biológica: Streptomyces hygroscopicus
• Nível de qualidade: 200
• Formulário: lyophilized powder
• purificado por: ion-exchange chromatography
• cor: faintly brown to brown; white to beige
• solubilidade: H₂O: 50 mg/mL
• espectro de atividade do antibiótico: viruses
• Modo de ação: protein synthesis | interferes
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• chave InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descrição

Descrição geral

Chemical structure: aminoglycoside

Aplicação

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes..

Ações bioquímicas/fisiológicas

Modo de ação: O produto age inibindo a síntese proteica induzindo a leitura errada do molde de mRNA em procariotos, com o potencial de inibir a tradução.

Espectro da ação antimicrobiana: A higromicina B age contra bactérias, fungos e células eucariotas superiores.

Atenção

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Nota de preparo

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Informações de segurança

• Pictogramas: GHS06
• Palavra indicadora: Danger
• Frases de perigo: H300 + H310 + H330
• Declarações de precaução: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Classificações de perigo: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable