H5527

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O, γ-irradiated

• Sinônimo(s): Hygromycin b, Hygrovetine
• Fórmula empírica (Notação de Hill): C₂₀H₃₇N₃O₁₃
• Número CAS: 31282-04-9
• Peso molecular: 527.52
• Beilstein: 6755837
• Número MDL: MFCD06795479
• Código UNSPSC: 51102829
• ID de substância PubChem: 24895698
• NACRES: NA.85

Propriedades

• fonte biológica: Streptomyces hygroscopicus
• Nível de qualidade: 200
• esterilidade: γ-irradiated
• concentração: ≥60% (HPLC); 45-60 mg/mL in H₂O
• cor: yellow-brown to orange
• espectro de atividade do antibiótico: viruses
• Modo de ação: protein synthesis | interferes
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• chave InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descrição

Descrição geral

Chemical structure: aminoglycoside

Aplicação

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Ações bioquímicas/fisiológicas

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Atenção

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Nota de preparo

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H₂O, and γ-irradiated .

Informações de segurança

• Pictogramas: GHS06,GHS08,GHS05
• Palavra indicadora: Danger
• Frases de perigo: H301 + H311,H318,H330,H334
• Declarações de precaução: P260 - P280 - P284 - P301 + P310 - P305 + P351 + P338 - P310
• Classificações de perigo: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter