Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

GE17-5442-02

Ficoll®-Paque Premium

Cytiva 17-5442-02, pack of 6 × 100 mL

Sinônimo(s):

Ficoll density gradient medium, cell culture media

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Código UNSPSC:
12352201
NACRES:
NA.25

forma

liquid

embalagem

pack of 6 × 100 mL

fabricante/nome comercial

Cytiva 17-5442-02

cor

Colorless to slightly yellow

faixa de pH

5.5-7.5

Categorias relacionadas

Descrição geral

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Aplicação

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Características e benefícios

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Ficoll is a registered trademark of Cytiva

Código de classe de armazenamento

12 - Non Combustible Liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Xin Ni et al.
PloS one, 12(9), e0184773-e0184773 (2017-09-15)
Inflammation has been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Connexins (Cxs)-based gap junction channels (GJCs) or hemichannels (HCs) are involved in the maintenance of homeostasis in the immune system. However, the
Avanish K Varshney et al.
PloS one, 13(1), e0190537-e0190537 (2018-01-25)
Staphylococcus aureus can cause devastating and life-threatening infections. With the increase in multidrug resistant strains, novel therapies are needed. Limited success with active and passive immunization strategies have been attributed to S. aureus immune evasion. Here, we report on a

Artigos

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocolos

How to make and use gradients of Percoll

How to use percoll to prepare a gradient

Conteúdo relacionado

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica