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10831

Sigma-Aldrich

Histopaque®-1083

sterile-filtered, density: 1.083

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

esterilidade

sterile-filtered

Nível de qualidade

Formulário

liquid

prazo de validade

Expiry date on the label.

Impurezas

endotoxin, tested

densidade

1.083

aplicação(ões)

hematology
histology

temperatura de armazenamento

2-8°C

Descrição geral

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Aplicação

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Características e benefícios

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Princípio

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Informações legais

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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