Pular para o conteúdo
Merck
Todas as fotos(2)

Documentos

GE17-0405-01

HiTrap® Protein G High Performance

Cytiva 17-0405-01, pack of 1 × 5 mL

Sinônimo(s):

Protein G affinity column, Protein purification column

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

embalagem

pack of 1 × 5 mL

fabricante/nome comercial

Cytiva 17-0405-01

condição de armazenamento

(20% Ehtanol)
 (20% Ethanol)

Parâmetros

flow rate
42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

2-9

working range

3-9

capacidade

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 125 mg binding capacity(human IgG/column)

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características e benefícios

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Artigos

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Ver tudo

Protocolos

Purification of Mouse Monoclonal IgG1 using HiTrap Protein

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica