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GE17-5111-01

HiTrap® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

embalagem

pack of 1 × 5 mL

fabricante/nome comercial

Cytiva 17-5111-01

condição de armazenamento

(20% Ethanol)

Parâmetros

<20 mL/min flow rate
42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

2-13

working range

3-11

temperatura de armazenamento

2-30°C

Descrição geral

HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

Aplicação

HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Características e benefícios

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
  • Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose High Performance.
  • Recovery and purity of IgY are excellent.
  • Can be operated with a liquid chromatography system such as ÄKTA® design.
  • The top and bottom frits are manufactured from porous polyethylene
  • Connectors for usage with different chromatography systems and other equipment are included

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

This page shows how to prepare samples for purification with affinity chromatography.

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