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Documentos Principais

A9046

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti Chicken Igy

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

rabbit

Nível de qualidade

conjugado

peroxidase conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

reatividade de espécies

chicken

técnica(s)

direct ELISA: 1:30,000
dot blot: 1:160,000 (indirect chemiluminescence)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.

Imunogênio

purified chicken IgG

Aplicação

Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.

Ações bioquímicas/fisiológicas

Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Nota de preparo

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Galvan A, et al.
International Journal of Cancer. Journal International Du Cancer, 136(5), E262-E271 (2015)
Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil
Oliveira A, et al,
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 27(2), 141-145 (2018)
Aline Sardinha-Silva et al.
PLoS pathogens, 15(6), e1007871-e1007871 (2019-06-22)
Infection of host cells by Toxoplasma gondii is an active process, which is regulated by secretion of microneme (MICs) and rhoptry proteins (ROPs and RONs) from specialized organelles in the apical pole of the parasite. MIC1, MIC4 and MIC6 assemble
Dae In Kim et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(24), E2453-E2461 (2014-06-14)
Proximity-dependent biotin identification (BioID) is a method for identifying protein associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest expressed in living cells, BioID permits the labeling of proximate proteins during a defined
Production and purification of IgY antibodies from chicken egg yolk.
Amro W A, et al.
Journal of Genetic Engineering and Biotechnology, 16(1), 99-103 (2018)

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