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Documentos Principais

AC146

Sigma-Aldrich

Normal Chicken IgY Antibody, purified | AC146

1 mg/mL, Chemicon®

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

chicken

Nível de qualidade

forma do anticorpo

purified antibody

clone

polyclonal

fabricante/nome comercial

Chemicon®

concentração

1 mg/mL

técnica(s)

blocking: suitable (interfering antibodies)

Isotipo

IgY

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week′s eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.

Aplicação

Gel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.

Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.

Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.
Research Category
Secondary & Control Antibodies
Research Sub Category
Isotype Control Antibodies

Qualidade

Evaluated by Western Blotting using non-reduced Chicken IgY.

Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.

Descrição-alvo

~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

forma física

Format: Purified
Purified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.
T-gel

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Emile Rage et al.
Applied microbiology and biotechnology, 103(18), 7491-7504 (2019-07-25)
Infectious bursal disease virus (IBDV) is the cause of an economically important highly contagious disease of poultry, and vaccines are regarded as the most beneficial interventions for its prevention. In this study, plants were used to produce a recombinant chimeric
Nealia Cm House et al.
eLife, 8 (2019-12-06)
CAG/CTG trinuncleotide repeats are fragile sequences that when expanded form DNA secondary structures and cause human disease. We evaluated CAG/CTG repeat stability and repair outcomes in histone H2 mutants in S. cerevisiae. Although the two copies of H2A are nearly

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