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GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

Sinônimo(s):

HiTrap Protein G, Protein G affinity column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Formulário

prepacked column

reticulação

cross-linked

embalagem

pack of 2 × 1 mL

fabricante/nome comercial

Cytiva 17-0404-03

condição de armazenamento

(20% Ehtanol)
 (20% Ethanol)

Parâmetros

42 psi

técnica(s)

liquid chromatography (LC): suitable

tamanho do leito

7 mm × 25 mm

volume do leito

1 mL

D.I. da coluna

7 mm

tamanho

Matriz

(cross-linked agarose, spherical)

Grupo ativo da matriz

Protein G

Tamanho médio da partícula

34 μm

cleaning

2-9

Faixa de trabalho

3-9

capacidade

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

técnica de separação

affinity

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características e benefícios

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

Lamentamos, não temos COA para este produto disponíveis online no momento.

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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

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This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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Protocolos

Purification of Mouse Monoclonal IgG1 using HiTrap Protein

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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