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Key Documents

GE28-9031-34

Protein G High Performance Spintrap

Cytiva 28-9031-34, pack of 16 columns

Sinônimo(s):

Protein G Column

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

Características

wetted part: no

embalagem

pack of 16 columns

fabricante/nome comercial

Cytiva 28-9031-34

condição de armazenamento

(20% Ehtanol)
(20% Ethanol)

matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

2-9

working range

3-9

capacidade

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(human IgG/column)

temperatura de armazenamento

2-8°C

Descrição geral

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap is a prepacked, single-use spin column containing protein G Sepharose High Performance.

Aplicação

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Características e benefícios

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocolos

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

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