C9281
Cholesterol Esterase from Pseudomonas fluorescens
lyophilized powder, ≥10,000 units/g protein
Sinônimo(s):
CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
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About This Item
Produtos recomendados
fonte biológica
Pseudomonas fluorescens
Nível de qualidade
Ensaio
10-30% (TCA-Biuret)
Formulário
lyophilized powder
atividade específica
≥10,000 units/g protein
peso molecular
~129 kDa
composição
Protein, ~20%
condição de armazenamento
under inert gas (argon)
técnica(s)
cell based assay: suitable
cor
tan to brown
pH
7-9
solubilidade
0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
temperatura de armazenamento
−20°C
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Descrição geral
Research area: Cell Signaling
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Aplicação
Cholesterol esterase from Pseudomonas fluorescens has been used in:
- in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
- a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
- an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
- for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
- to hydrolyze native cholesterol ester (CE) during filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.
Ações bioquímicas/fisiológicas
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..
Outras notas
Contains potassium phosphate and TRITON® X-100.
Definição da unidade
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.
Nota de análise
Protein determined by biuret.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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