COLLH-RO

Collagenase H

from Clostridium histolyticum

• Número da licença da enzima: 3.4.24.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: Clostridium histolyticum
• Nível de qualidade: 100
• esterilidade: non-sterile
• Formulário: lyophilized
• collagenase activity: 0.15 U/mg lyophilizate
• embalagem: pkg of 100 mg (11074032001); pkg of 2.5 g (11087789001); pkg of 500 mg (11074059001)
• fabricante/nome comercial: Roche
• concentração: ~1 mg/mL (Isolation of rat hepatocytes); ~2 mg/mL (Isolation of adipocytes)
• técnica(s): tissue processing: suitable
• cor: brown
• pH ideal: 6.0-8.0
• solubilidade: water: soluble
• nº de adesão NCBI: WP_138210331
• nº de adesão UniProt: Q46085
• aplicação(ões): life science and biopharma
• atividade externa: Clostripain 39.798 U/mg; Proteases 14.014 U/mg (Azocoll, based on lyoph); Trypsin 1.668 U/mg (with BAEE)
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Collagenase H is an enzyme mixture prepared from Clostridium histolyticum cultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Especificidade

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Aplicação

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

forma física

Lyophilizate, nonsterile

Nota de preparo

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator: Ca²⁺
Working concentration: 0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

Reconstituição

Reconstitution in any balanced salt solution (e.g., HBSS)

Armazenamento e estabilidade

Keep container tightly closed in a dry and well-ventilated place.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS07,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H315 - H319 - H334 - H335
• Declarações de precaução: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash