ABT1487
Anti-α-Smooth Muscle Actin (ACTA2) Antibody,
rabbit polyclonal
Sinônimo(s):
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Nome do produto
Anti-alpha-Actin Antibody, Smooth Muscle, from rabbit, purified by affinity chromatography
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
rat, mouse, human
reatividade da espécie (prevista por homologia)
chicken (based on 100% sequence homology)
técnica(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ACTA1(58)
Descrição geral
Actin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
Especificidade
This polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3).
Imunogênio
Epitope: N-terminus.
KLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin.
Aplicação
Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation.
Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot immunostained smooth muscle alpha-Actin in NIH/3T3 fibroblasts, HUVECs, A431 and HeLa cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Qualidade
Evaluated by Western Blotting in C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate.
Descrição-alvo
~42 kDa observed.
forma física
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Outras notas
Concentration: Please refer to lot specific datasheet.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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