437627

Lipopolysaccharide, E. coli O111: B4

from E. coli, , lyophilized, iNOS stimulator, Calbiochem^®

• Sinônimo(s): E. coli Lipopolysaccharide, E. coli O111:B4 LPS
• Código UNSPSC: 12352201
• NACRES: NA.77

Propriedades

• Nome do produto: Lipopolysaccharide, E. coli O111: B4, Lipopolysaccharide, E. coli O111: B4 stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes
• Nível de qualidade: 100
• descrição: RTECS - OJ0895400
• Formulário: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Impurezas: ≤2.0% Protein; ≤2.5% nucleic acid
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Endotoxin lipopolysaccharides initiate a variety of biochemical cell activation pathways (PKC, cAMP-dependent protein kinase, arachidonate metabolism, protein myristoylation, and activation of G-proteins). These unique macromolecules have been extensively studied in order to elucidate and define relevant pathophysiological parameters of endotoxic shock, a profound life-threatening consequence of sepsis. More recently, these bacterial products have generated intense interest due to their pluropotential immunostimulatory activity as manifested by the activation of host cells (e.g. B lymphocytes, macrophages) to functional differentiation. Host cell activation by lipopolysaccharides produces a spectrum of hormone-active lymphokines and monokines, including interferons (α, β, and γ), interleukins (IL-1 and IL-6), tumor necrosis factor, platelet activating factor, and procoagulant tissue factor. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
Inducible nitric oxide synthase

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Harmful (C)

Nota de preparo

To achieve complete solubilization, heating solution to 37°C with intermittent vortexing or sonication may be necessary.

Reconstituição

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Outras notas

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int. 35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol. 49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem. 60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.
Jackson, S.K., et al. 1992. Biochim. Biophys. Acta1135, 165.
O′Sullivan, M.G., et al. 1992. J. Biol. Chem.267, 14547.
Watanabe, K., et al. 1992. J. Cell. Physiol.150, 433.
Morrison, D.C., and Ryan, J.L. 1987. Annu. Rev. Med.38, 417.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS06
• Palavra indicadora: Danger
• Frases de perigo: H300
• Declarações de precaução: P264 - P270 - P301 + P310 - P405 - P501
• Classificações de perigo: Acute Tox. 2 Oral
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable