16-222

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate

clone 3H10, Upstate^®, from mouse

• Sinônimo(s): H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: FITC conjugate
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 3H10, monoclonal
• reatividade de espécies: human
• fabricante/nome comercial: Upstate^®
• técnica(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_003484
• nº de adesão UniProt: Q16695
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer10)
• Informações sobre genes: human ... HIST1H3F(8968)

Descrição

Descrição geral

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidade

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

Imunogênio

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Epitope: Ser10

Aplicação

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

Qualidade

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.

Descrição-alvo

17 kDa

forma física

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

Armazenamento e estabilidade

Stable for 1 year at from date of receipt.

Nota de análise

Control
Mitotic HeLa cells (IF).

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2