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MilliporeSigma

R7644

Sigma-Aldrich

Transcript RNA Markers 0.28-6.6 kb

for RNA electrophoresis

Synonym(s):

0.28-6.6 kb Transcript RNA Markers, Transcript RNA markers for RNA electrophoresis

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

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This Item
R7020D2916D9281
Quality Level

200

Quality Level

300

Quality Level

100, 200

Quality Level

100, 200

form

liquid

form

liquid

form

buffered aqueous solution

form

liquid

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

usage

25 uses

usage

25 uses

usage

100 uses (0.1 mg size), 20 uses (20 μg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (RNA)

suitability

suitable for electrophoresis (RNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) contains 9 RNA transcripts from 281 – 6,583 bases. Three μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Transcript RNAMarkers 0.28-6.6 kb are suitable for use as an RNA ladder for formaldehyde agarose gel electrophoresis.[1] It is also suitable for the size determination of small RNA using native or denaturing agarose gel electrophoresis.[2]

Components

Sigma′s Transcript RNA Marker (0.28 – 6.6 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains a mixture of nine RNA transcripts, 281–6,583 bases
For optimal resolution, the recommended agarose gel concentration is 1.0%.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Detection of two mRNA species at single-cell resolution by double-fluorescence in situ hybridization.
Pinaud R, et al.
Nature Protocols, 3, 1370-1379 (2008)

Articles

Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.

Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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