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G2526

Sigma-Aldrich

Gel Loading Buffer

for NA electrophoresis

Quality Level

grade

for molecular biology

form

liquid

foreign activity

RNAse, none detected

storage temp.

room temp

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General description

Gel loading buffer is used as a tracking dye during electrophoresis. The dye has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample before loading.

Application

Gel Loading Buffer has been used for loading polymerase chain reaction (PCR) amplified intergenic spacer region (ISR) sequence samples, PCR amplified DNA samples of the intestinal mucosa, Trypanosoma sp DNA on agarose gel for electrophoresis.It is suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.

Biochem/physiol Actions

Gel loading buffer is especially formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids. The gel loading solution contains a bromophenol blue tracking dye and sucrose to add density and facilitate sample loading. EDTA has been included to inhibit nucleases that require divalent cations and SDS has been added to help dissociate DNA-protein complexes which can interfere with electrophoresis.

Components

Gel loading buffer contains 0.05% bromophenol blue , 40% sucrose, 0.1M EDTA (pH 8.0) and 0.5% SDS.

Other Notes

If crystals form, dissolve them by heating the solution to 65C for 10 minutes.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Validation of a polymerase chain reaction assay for monitoring the therapeutic efficacy of diminazene aceturate in trypanosome-infected sheep
Bengaly Z, et al.
Veterinary Parasitology, 96(2), 101-113 (2001)
Comparison of diagnostic techniques for porcine proliferative enteropathy (Lawsonia intracellularis infection)
Huerta B, et al.
Journal of Comparative Pathology, 129(2-3), 179-185 (2003)
Identification of dairy lactic acid bacteria by tRNAAla-23S rDNA-RFLP
Mancini A, et al.
Journal of Microbiological Methods, 91(3), 380-390 (2012)

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