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PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution


Quality Level



storage temp.

room temp

Related Categories

General description

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.


Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Tao Qin et al.
Plant physiology, 175(3), 1321-1336 (2017-09-10)
Long noncoding RNAs (lncRNAs) affect gene expression through a wide range of mechanisms and are considered as important regulators in many essential biological processes. A large number of lncRNA transcripts have been predicted or identified in plants in recent years....
Angel Emilio Martínez de Alba et al.
Nucleic acids research, 39(21), 9339-9344 (2011-08-05)
In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous...
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin...
Alavala Matta Reddy et al.
BMC genomics, 10, 65-65 (2009-02-07)
MicroRNAs (miRNAs) are small ~22-nt regulatory RNAs that can silence target genes, by blocking their protein production or degrading the mRNAs. Pig is an important animal in the agriculture industry because of its utility in the meat production. Besides, pig...
Xianfeng Morgan Xu et al.
The Plant cell, 19(5), 1537-1548 (2007-05-22)
Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis thaliana NUCLEAR PORE ANCHOR (NUA) encoding a...


Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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