All Photos(1)

S7025

Sigma-Aldrich

50 bp DNA Step Ladder

for DNA electrophoresis

NACRES:
NA.25

Quality Level

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Components

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Other Notes

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Rhodotorula portillonensis sp. nov., a basidiomycetous yeast isolated from Antarctic shallow-water marine sediment.
Laich F, et al.
International Journal of Systematic and Evolutionary Microbiology, 63, 3884-3891 (2013)
Alper Dede et al.
Microbial pathogenesis, 143, 104134-104134 (2020-03-15)
Soil actinomycetes are a highly common group of bacteria and frequently studied as having secondary metabolites in the potential of producing the most preferred antagonistic content. Considering the continuous variation in soil structure, there is a potential for encountering different...

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service