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D9780

Sigma-Aldrich

Lambda DNA Hind III Digest

for DNA electrophoresis

NACRES:
NA.25

Quality Level

grade

for molecular biology

form

liquid

usage

100 uses (0.1 mg size)
1000 uses (1 mg size)
20 uses (20 μg size)
500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

shipped in

dry ice

storage temp.

−20°C

General description

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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