MilliporeSigma
All Photos(3)

D9780

Sigma-Aldrich

Lambda DNA Hind III Digest

for DNA electrophoresis

All Photos(3)
NACRES:
NA.25

grade

for molecular biology

Quality Level

100
200

form

liquid

usage

100 uses (0.1 mg size)
1000 uses (1 mg size)
20 uses (20 μg size)
500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

shipped in

dry ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
D9281D0672D6293
Lambda DNA Hind III Digest for DNA electrophoresis

Sigma-Aldrich

D9780

Lambda DNA Hind III Digest

Lambda DNA EcoR I Hind III Digest for DNA electrophoresis

Sigma-Aldrich

D9281

Lambda DNA EcoR I Hind III Digest

Sigma-Aldrich

Sigma-Aldrich

D0672

φX174 DNA Marker Hae III Digest

pUC18 DNA Hae III Digest for DNA electrophoresis

Sigma-Aldrich

D6293

pUC18 DNA Hae III Digest

grade

for molecular biology

grade

-

grade

-

grade

-

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

usage

100 uses (0.1 mg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

General description

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service