S9430

SYBR^® Green I nucleic acid gel stain

10,000 × in DMSO

• Synonym(s): safer gel stain, DNA stain, SYBR green gel dye
• CAS Number: 163795-75-3
• MDL number: MFCD00284715
• NACRES: NA.52

Properties

• Quality Level: 200
• usage: mL sufficient for 100 mini-gels
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• concentration: 10,000 × in DMSO
• greener alternative category: Aligned
• storage temp.: −20°C

Description

General description

SYBR^® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Application

SYBR^® Green I nucleic acid gel stain has been used for:

• the quantification of dsDNA
• to stain DNA in polymerase chain reaction (PCR)
• for comet assay technique
• to assess spermatozoon membrane integrity
• for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
• as a fluorescent dye in flow cytometry
• real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Features and Benefits

• An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
• It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
• It is less mutagenic than ethidium bromide in Ames tests
• It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
• Useful for many applications with a limited amount of DNA
• The binding of SYBR^® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
• Removal of this stain in-gel digestion and ligation techniques is not needed
• SYBR Green I is a greener alternative product to ethidium bromide for staining

Storage and Stability

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Legal Information

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

SYBR is a registered trademark of Life Technologies

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 201.2 °F - closed cup
• Flash Point(C): 94 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves