QR0100

SYBR^® Green Quantitative RT-qPCR Kit

One step SYBR^® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

• Synonym(s): one step rt qPCR, sybr green qPCR
• NACRES: NA.55

Properties

• Quality Level: 200
• usage: sufficient for 250 reactions
• feature: dNTPs included; hotstart
• technique(s): RT-qPCR: suitable
• color: colorless
• input: purified RNA
• compatibility: ABI 5700; ABI 7000; ABI 7300; ABI 7500 Fast; ABI 7500; ABI 7700; ABI 7900 HT ; ABI 7900 HT Fast; ABI 7900; ABI StepOne; ABI StepOnePlus; ABI ViiA 7; Bio-Rad CFX384; Bio-Rad CFX96; Bio-Rad MJ Chromo4; Bio-Rad MJ Opticon 2; Bio-Rad MJ Opticon; Bio-Rad MiniOpticon; Bio-Rad MyiQ; Bio-Rad iCycler iQ; Bio-Rad iQ5; Cepheid SmartCycler; Eppendorf^® Mastercycler ep realplex2 s; Eppendorf^® Mastercycler ep realplex; Illumina Eco qPCR; Qiagen Corbett Rotor-Gene 3000; Qiagen Corbett Rotor-Gene 6000; Qiagen Corbett Rotor-Gene Q; Roche LightCycler 480; Strategene Mx3000P; Strategene Mx3005P; Strategene Mx4000
• detection method: SYBR^® Green
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The SYBR^® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart^™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

SYBR^® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay:

• to detect specific genetic clusters of genogroup I and II noroviruses

[93]

for chikungunya viral (CHIKV) RNA quantification

[94]

to detect mRNA expression levels

[92][55]

for amplification of total RNA extracted from human umbilical vein endothelial cells (HUVECs)

[71] and prostate cancer cells

Features and Benefits

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced preparation time and the risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot Start methods
• JumpStart^™Taq Polymerase reduces primer-dimer and non-specific product formation
• SYBR^® Green I dye is inexpensive, easy to use, and highly sensitive
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 100 reactions at 50 μL each

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Kit Components Only

• SYBR^® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25

Kit Components Also Available Separately

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included
• M8787 25 mM MgCl₂ 1.5 mL/vial
• R4526 Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vial

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H314 - H317 - H410
• Precautionary Statements: P261 - P272 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable