QR0100
sufficient for 250 reactions
dNTPs included
hotstart
RT-qPCR: suitable
colorless
purified RNA
ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
SYBR® Green
wet ice
−20°C
Danger
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
8A - Combustible, corrosive hazardous materials
WGK 3
Not applicable
Not applicable
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RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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