R4526

Reference Dye for Quantitative PCR

100 ×, solution

• Synonym(s): ROX
• NACRES: NA.55

Properties

• form: solution
• Quality Level: 300
• usage: sufficient for ≥600 reactions
• concentration: 100 ×
• color: red
• storage temp.: 2-8°C

Description

General description

Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:

• in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
• as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
• for analyzing the degree of cellular DNA contamination by qPCR

Packaging

0.3, 5 mL

20 reactions

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H315 - H317 - H319 - H410
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves