MilliporeSigma
All Photos(1)

R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):
ROX
NACRES:
NA.55

Quality Level

form

solution

usage

sufficient for ≥600 reactions

concentration

100 ×

color

red

storage temp.

2-8°C

General description

Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Packaging

0.3 mL in poly bottle

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Rathindra N Bose et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(47), 18314-18319 (2008-11-21)
DNA is believed to be the molecular target for the cytotoxic activities of platinum (Pt) anticancer drugs. We report here a class of platinum(II)- and platinum(IV)-pyrophosphato complexes that exhibit cytotoxicity comparable with and, in some cases, better than cisplatin in
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Anja Nilsen et al.
Translational oncology, 12(3), 576-584 (2019-01-21)
MicroRNA (miRNA) expressions in tumor biopsies have shown potential as biomarkers in cervical cancer, but suitable reference RNAs for normalization of reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays in patient cohorts with different clinicopathological characteristics are not available. We
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)

Protocols

sybr green jumpstart taq readymix

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service