MilliporeSigma
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W1754

Sigma-Aldrich

Water

PCR Reagent

Synonym(s):
H2O
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

PCR Reagent

Quality Level

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected
RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Water for molecular biology, sterile filtered

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Water

grade

PCR Reagent

grade

-

grade

ACS reagent

grade

for molecular biology

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

-

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

3 mmHg

vapor pressure

-

form

liquid

form

-

form

liquid

form

liquid

technique(s)

PCR: suitable

technique(s)

-

technique(s)

-

technique(s)

FISH: suitable

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR).
Water has been used:
  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA

Suitability

Suitable for polymerase chain reaction (PCR)

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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