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270733

Sigma-Aldrich

Water

suitable for HPLC

Synonym(s):

Aqua, Dihydrogen oxide

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein/REAXYS Number:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
12190000
PubChem Substance ID:
329752968
NACRES:
NA.21

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.

Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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