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R7020

Sigma-Aldrich

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

NACRES:
NA.25

Quality Level

grade

for molecular biology

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Components

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Carc. 1B - Muta. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch.
Wright SA, et al.
Plant Pathology, 62, 982-992 (2013)

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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