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T5941

Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Synonym(s):

TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
UNSPSC Code:
12161700
PubChem Substance ID:
24900332
NACRES:
NA.25

Quality Level

400

grade

BioPerformance Certified

assay

≥99.0% (titration)

form

crystalline powder

technique(s)

DNA purification: suitable
cell culture | mammalian: suitable
electrophoresis: suitable
protein extraction: suitable

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
 endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 667 mg/mL

density

1.39 g/cm3 at 20—25 °C
1.40 g/cm3 at 20—25 °C

absorption

≤0.05 at 290 at 40%

suitability

suitable for electrophoresis
suitable for immunohistochemistry
suitable for molecular biology

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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General description

Tris HCl, also known as Trizma® hydrochloride, is a widely used acidic buffer for preparing buffers within the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, Tris HCl offers a pH range of 7.0 to 9.0, matching the typical pH of living organisms. As a result, Tris HCl is an essential component of buffer solutions used in various biological, cell culture, biochemistry, and molecular biology applications. It is important to note that neither Tris base nor Tris hydrochloride on their own can provide sufficient buffering capacity. Therefore, they are typically combined to create a buffer within the pH range of 7 to 9, ensuring effective pH control.

Tris buffers are indispensable in techniques like protein electrophoresis and western blotting. In many western blot protocols, a low ionic strength Tris buffer is employed for protein transfer. The transfer duration depends on the blotting apparatus and the size range of peptides under investigation. Tris-based buffers are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers.

Furthermore, Tris salts find extensive use in DNA agarose electrophoresis, particularly in the preparation of buffers like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA). These buffers play a pivotal role in various applications, including biochemistry, protein purification, and electrophoresis. Tris HCl is a versatile and essential for maintaining the pH of biological systems and ensuring the optimal performance of biochemical, cell culture, and molecular biology techniques.

Application

Tris-HCl hasbenn used
  • as a washing and rinsing buffer in an immunohistochemical analysis(1)
  • as a component of reducing sample buffer, lysis buffer and elution buffer, in a biochemical research involving gel electrophoresis and BioLayer interferometry measurement(2)

Features and Benefits

  • Suitable for Molecular Biology and Cell Culture
  • Can be used as a Buffer component, for Electrophoresis and Protein separation
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, and Protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
  • Highly soluble in water

Packaging

T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methods in enzymology, 182, 147-153 (1990-01-01)
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