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Solid-phase hybridization efficiency improvement on the magnetic nanoparticle surface by using dextran as molecular arms.

Journal of biomedical nanotechnology (2013-09-26)
Zhiyang Li, Haowen Yang, Nongyue He, Wenbiao Liang, Chao Ma, Muhammad Ali A Shah, Yongjun Tang, Song Li, Hongna Liu, Hesheng Jiang, Yafen Guo
ABSTRACT

For reducing the steric hindrance and nonspecific binding of the target DNA, the dextran was used as molecular arms to be immobilized on the surface of magnetic nanoparticles (MNPs). Magnetic separation was used in preparation of dextran-MNPs (DMNPs). Aspartic acid and aminated DNA probe were successively modified on the dextran immobilized on the surface of MNPs. These probe-DMNPs were successfully applied to detect biotin-labeled PCR product of E. coli O157:H7 genome by hybridization. Then the complexes were bonded with streptavidin-modified alkaline phosphatase (ALP-SA). Finally the chemiluminescent signals were detected by adding 3-(2-spiroadamantane)-4-methoxy-4- (3-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD). The results showed that this method had a good specificity, and higher sensitivity than that when only MNPs were used as solid carriers.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 60,000-76,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 150,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 9,000-11,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 48,000-90,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
Sigma-Aldrich
Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 12,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 1,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 25,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~40,000
Sigma-Aldrich
Dextran, enzymatic synth.
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~6,000
Supelco
Dextran, analytical standard, for GPC, Set Mp 1,000-400,000
Supelco
Dextran, analytical standard, for GPC, 5,000
Supelco
Dextran, analytical standard, for GPC, 1,000
Supelco
Dextran, analytical standard, for GPC, 150,000
Supelco
Dextran, analytical standard, for GPC, 670,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 15,000-25,000
Supelco
Dextran, analytical standard, for GPC, 25,000
Supelco
Dextran, analytical standard, for GPC, 50,000
Supelco
Dextran, analytical standard, for GPC, 270,000
Supelco
Dextran, analytical standard, for GPC, 80,000
Supelco
Dextran, analytical standard, for GPC, 410,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, Mr ~200,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 410,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 450,000-650,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~70,000