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T9179

Sigma-Aldrich

Terrific Broth

EZMix powder microbial growth medium

Synonym(s):
TB media
NACRES:
NA.85

grade

for molecular biology

Quality Level

description

TB; quick dissolve, dust-free formulation

sterility

non-sterile

form

powder

application(s)

agriculture
food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
  • as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
[89]
  • to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
  • [91]
  • Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
  • [58]

    Packaging

    1 kg in poly bottle

    Features and Benefits

    Terrific Broth EZMix powder provides:
    • Granulated, dust-free format for safer handling and faster mixing
    • A budget-friendly alternative to liquid
    • Modified formulation (without glycerol)
    • Easy scale-up using larger package sizes

    Components

    *Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

    23.6g/L Yeast Extract
    11.8g/L Tryptone
    9.4g/L K2HPO4
    2.2g/L KH2PO4

    Reconstitution

    Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

    Legal Information

    EZMix is a trademark of Merck KGaA, Darmstadt, Germany

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

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