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CMC0017

Sigma-Aldrich

OverExpress™ C41(DE3) Chemically Competent Cells

for the highest protein expression

All Photos(6)
Synonym(s):
C41 strain
NACRES:
NA.85

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OverExpress™ C43(DE3) Chemically Competent Cells for the highest protein expression

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CMC0019

OverExpress™ C43(DE3) Chemically Competent Cells

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OverExpress™ C41(DE3) pLysS Chemically Competent Cells for the highest protein expression

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OverExpress™ C43(DE3) pLysS Chemically Competent Cells for the highest protein expression

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OverExpress™ C43(DE3) pLysS Chemically Competent Cells

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Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

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70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

BL21(DE3) Electrocompetent Cells for protein expression

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CMC0016

BL21(DE3) Electrocompetent Cells

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BL21(DE3) pLysE Chemically Competent Cells for protein expression

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BL21(DE3) pLysE Chemically Competent Cells

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Rosetta-gami B(DE3) Competent Cells - Novagen Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

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Rosetta-gami B(DE3) Competent Cells - Novagen

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Origami 2(DE3) Competent Cells - Novagen Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

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71345-M

Origami 2(DE3) Competent Cells - Novagen

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Tuner (DE3) Competent Cells - Novagen® Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

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70623

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Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

OverExpress™ C41 (DE3) chemically competent cells have been used for expression screening.

Packaging

40 μL

Features and Benefits

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Components

  • OverExpress C41(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Legal Information

OverExpress is a trademark of Imaxio, S.A.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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