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CMC0017

Sigma-Aldrich

OverExpress C41(DE3) Chemically Competent Cells

for the highest protein expression

NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

OverExpress C41 (DE3) chemically competent cells have been used for expression screening.

Features and Benefits

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Components

  • OverExpress C41(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Legal Information

OverExpress is a trademark of Imaxio, S.A.

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

A high-throughput expression screening platform to optimize the production of antimicrobial peptides.
Schreiber C, et al.
Microbial cell factories, 16(1), 29-29 (2017)
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair....
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in...
Myles B C Dillon et al.
The Journal of biological chemistry, 288(39), 27872-27880 (2013-08-16)
Protein arginine methyltransferase (PRMT) 8 is unique among the PRMTs, as it has a highly restricted tissue expression pattern and an N terminus that contains two automethylation sites and a myristoylation site. PRMTs catalyze the transfer of a methyl group...
Neale Harrison et al.
PloS one, 9(11), e113007-e113007 (2014-11-26)
N-acylethanolamines are an important class of lipid signaling molecules found in many species, including the nematode Caenorhabditis elegans (C. elegans) where they are involved in development and adult lifespan. In mammals, the relative activity of the biosynthetic enzyme N-acyl phosphatidylethanolamine-specific...

Protocols

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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