for molecular biology
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
10 - Combustible liquids
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Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.