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CMC0015

Sigma-Aldrich

BL21(DE3) pLysE Chemically Competent Cells

for protein expression

NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) pLysE chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

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