for molecular biology
buffered aqueous solution
competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg
10 - Combustible liquids
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For best results, ligation reactions must be heat inactivated at 70ºC for 15 minutes before transformation. Alternately, the reactions may be purified.
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.