MilliporeSigma
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CMC0001

Sigma-Aldrich

SIG10 Chemically Competent Cells

for protein expression and DNA plasmid production

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NACRES:
NA.85

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Recovery Medium for Cloning for use with competent cells

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for use with competent cells

BL21(DE3) Competent Cells - Novagen BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

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NovaBlue Competent Cells - Novagen NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

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NovaBlue Competent Cells - Novagen

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

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SOC Medium

For use in transformation

OverExpress™ C41(DE3) Chemically Competent Cells for the highest protein expression

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OverExpress™ C41(DE3) Chemically Competent Cells

for the highest protein expression

grade

for molecular biology

form

buffered aqueous solution

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA

Packaging

1 ea
40, 40, 40 μL

Application

Suitable for bacterial transformations to recover high quality plasmid DNA

Features and Benefits

  • share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • ensures recovery of stable and high quality plasmid DNA.
  • high transformation efficiency
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Components

  • SIG10 chemically competent cells
  • pUC 19 transformation control DNA at 10 pg/μL
  • recovery medium for cloning

Principle

The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.

Legal Information

DH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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