MilliporeSigma
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CMC0007

Sigma-Aldrich

SIG10 5a Chemically Competent Cells

for DNA plasmid production

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NACRES:
NA.85

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Escherichia coli Strain K12, lyophilized cells

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CONTROLLER SIG10 Chemically Competent Cells for controlled protein expression

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for controlled protein expression

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LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

grade

for molecular biology

form

buffered aqueous solution

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 108 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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General description

The SIG10 5a Chemically Competent Cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols.
They provide the performance researchers need with ease of use as only common laboratory equipment is required and provide solutions for a wide range of applications at economical prices.

Genotype

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Packaging

40 μL

Features and Benefits

  • Direct replacements for standard cloning strains like DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.
  • Optimized genetics for high yields: endonuclease and recombination minus
  • Blue - white screening

Components

  • SIG10 5a chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Legal Information

DH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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