for molecular biology
competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg
10 - Combustible liquids
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Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
BL21(DE3) Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note that alternate settings result in transformation efficienes about 20-50% lower. Typical time constants are 3.5 to 4.5 msec.