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CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

for protein expression

NACRES:
NA.85

grade

for molecular biology

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Components

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as...
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows...

Protocols

BL21(DE3) Electrocompetent Cells

BL21(DE3) Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note that alternate settings result in transformation efficienes about 20-50% lower. Typical time constants are 3.5 to 4.5 msec.

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