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G5516

Sigma-Aldrich

Glycerol

for molecular biology, ≥99.0%

Synonym(s):
1,2,3-Propanetriol, Glycerin
Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
Molecular Weight:
92.09
Beilstein:
635685
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology
reagent

Quality Level

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

assay

≥99.0%

form

liquid

autoignition temp.

698 °F

technique(s)

MALDI-MS: suitable

refractive index

n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.25 g/mL (lit.)

cation traces

Fe: ≤5 ppm
Mg: ≤5 ppm
heavy metals (as Pb): <5 ppm

foreign activity

DNase, RNase, NICKase, and protease, none detected

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.[29][33]

Application

Glycerol has been used as:
  • A supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
[33]
  • A fuel during designing enzymatic biofuel cell.
  • [35]
  • A liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • [37][39]
  • A matrix for fast atom bombardment MS.
  • [37][39]
  • May be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
  • [40]
    Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
    Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

    Packaging

    100, 500 mL in poly bottle
    1, 4 L in poly bottle

    Biochem/physiol Actions

    Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.[29][33]

    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 1

    Flash Point(F)

    390.2 °F - Pensky-Martens closed cup

    Flash Point(C)

    199 °C - Pensky-Martens closed cup

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

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