for molecular biology
3.1 (vs air)
<1 mmHg ( 20 °C)
n20/D 1.474 (lit.)
182 °C/20 mmHg (lit.)
20 °C (lit.)
1.25 g/mL (lit.)
Fe: ≤5 ppm
Mg: ≤5 ppm
heavy metals (as Pb): <5 ppm
DNase, RNase, NICKase, and protease, none detected
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Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.