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R4653

Sigma-Aldrich

Monoclonal Anti-hnRNP-A2/B1 antibody produced in mouse

clone DP3B3, purified from hybridoma cell culture

Synonym(s):
Anti-Heterogeneous Nuclear Ribonucleoprotein-A2/B1
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DP3B3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~36 kDa

species reactivity

rat, hamster, mouse, human, canine, chicken, monkey, bovine

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract from 293T cell line

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Monoclonal Anti-hnRNP-A2/B1 (mouse IgG2a isotype) is derived from the DP3B3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human hnRNP-A2. hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells. However, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin and the supraoptic nucleus of the hypothalamus.

Immunogen

recombinant human hnRNP-A2.

Application

Monoclonal Anti-hnRNP-A2/B1 antibody produced in mouse been used in :
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

In cells, it actively participates in pre-messenger RNA (pre-mRNA) processing, mRNA metabolism and transportation. It is overexpressed in the initial stage of lung cancer and in premalignant lesions. It also participates in neurodegeneration mediated by rCGG (repeats).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Quotes and Ordering

Immunohistochemical study of the hnRNP A2 and B1 in the rat forebrain
Mizukami K, et al.
Neuroreport, 11(14), 3099-3102 (2000)
Amanda M Nelson et al.
The Journal of investigative dermatology, 126(10), 2178-2189 (2006-04-01)
Isotretinoin (13-cis retinoic acid (13-cis RA)) is the most potent inhibitor of sebum production, a key component in the pathophysiology of acne, yet its mechanism of action remains largely unknown. The effects of 13-cis RA, 9-cis retinoic acid (9-cis RA)
Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB
Aksenova V, et al.
Oncotarget, 4(2), 362-372 (2013)
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications
Kourtidis A and Anastasiadis PZ
Cell Cycle, 15(4), 498-505 (2016)
Rocio Bengoechea et al.
Human molecular genetics, 24(23), 6588-6602 (2015-09-13)
Limb-girdle muscular dystrophy type 1D (LGMD1D) is caused by dominantly inherited missense mutations in DNAJB6, an Hsp40 co-chaperone. LGMD1D muscle has rimmed vacuoles and inclusion bodies containing DNAJB6, Z-disc proteins and TDP-43. DNAJB6 is expressed as two isoforms; DNAJB6a and

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