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P4707

Sigma-Aldrich

Poly-L-lysine solution

0.01%, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
PLL Solution
CAS Number:
MDL number:
eCl@ss:
32160406
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Related Categories

Application

  • Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
[47]
  • Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
  • [48]
  • Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.
  • [49]
    Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

    Biochem/physiol Actions

    Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

    Components

    Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

    Preparation Note

    This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 2

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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    Product Information Sheet

    SDS

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