MilliporeSigma
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P8920

Sigma-Aldrich

Poly-L-lysine solution

0.1 % (w/v) in H2O

CAS Number:
MDL number:
eCl@ss:
32160406
NACRES:
NA.47

biological source

synthetic

Quality Level

shelf life

Expiry date on the label.

mol wt

150,000-300,000

concentration

0.1 % (w/v) in H2O

color

colorless

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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This Item
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Poly-L-lysine solution 0.1 % (w/v) in H2O

Sigma-Aldrich

P8920

Poly-L-lysine solution

Sigma-Aldrich

Sigma-Aldrich

P4707

Poly-L-lysine solution

Sigma-Aldrich

Sigma-Aldrich

P4832

Poly-L-lysine solution

biological source

synthetic

biological source

-

biological source

-

biological source

-

shelf life

Expiry date on the label.

shelf life

-

shelf life

-

shelf life

-

mol wt

150,000-300,000

mol wt

-

mol wt

150,000-300,000

mol wt

15,000-30,000

concentration

0.1 % (w/v) in H2O

concentration

0.01%

concentration

0.01%

concentration

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

Application

Poly-L-lysine solution has been used for the attachment of endothelial cell cross sections to UV-crosslinked PET membrane frame slides. Poly-L-lysine coated slides were used for the placement of tissue sections for immunohistochemical assay.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

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