pkg of 10 X 5 mg
pkg of 5 mg
cell culture | mammalian: suitable
H2O: soluble 50 mg/mL, clear, colorless
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The protocol for coating surfaces with poly-D-lysine is the same as for poly-L-lysine. The optimal conditions must be determined for each cell line and application. In general, the following steps can be used.1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-D-lysine.2. Aseptically coat culture surface with 0.5-1.0 ml of solution per 25 cm2. Rock gently to ensure even coating of the culture surface.3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.4. Allow to dry at least two hours before introducing cells and medium.
The hydrogen bromide will not interfere with the cell growth. The hydrogen bromide salts are used to make our poly-L-lysine solutions that are cell culture tested. For these products, there is no interference in cell growth from the bromide.
Certain cells can digest poly-L-lysine, in which case the poly-D-lysine should be used.
Poly-lysine is used to coat surfaces to increase the charge present. The lower molecular weight poly-L-lysine (30-70 kD) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kD) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kD. Please check the literature for information on the molecular weight range used for your cell type.
Poly-lysine solutions (0.1 to 1 mg/ml) can be sterile-filtered (0.2 um) and should be stored at 2-8 °C. Solutions should not be stored frozen as thawing can cause a decrease in the size (molecular weight range) of the polymer.
The same protocol can be followed for coating plates with poly-lysine. The transwell membrane is immersed in the poly-lysine solution.
Plates and/or slides can be stored after drying, protected from dust for up to 1 year. Inspect the plates/slides to make sure there are no cracks in the coating prior to use. Each lab should determine the maximum length of time for storage based on the application.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Ask a Scientist here.
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