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L2020

Sigma-Aldrich

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

Synonym(s):
Laminin
CAS Number:
MDL number:
NACRES:
NA.75

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Quality Level

product line

BioReagent

form

aqueous solution

mol wt

A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa

packaging

pkg of 1 mg

concentration

1-2 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

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Application

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:
  • insulin-producing cell (IPC) differentiation.
  • Infection inhibition assays.
  • Proliferation Assay.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Caution

It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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