pkg of 5 x 5 mg
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
cell culture | mammalian: suitable
H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
human ... FN1(2335)
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
The product is good for at least a year at 2-8 °C if kept sterile.
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.
We test the coated culture surface by culturing cells. We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive). The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.
As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance. The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
The difference between Product Nos. F2006 and F1056 is in the purity specifications and endotoxin testing. Product No. F2006 has a purity specification of ≥90% and is not tested for endotoxin contamination. Product No. F1056 has a purity specification of ≥95% and is tested for endotoxin contamination with a specification of ≤100 EU/mg.
Ask a Scientist here.
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.