pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
cell culture | mammalian: suitable
bovine ... FN1(280794)
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
The product is good for at least a year at 2-8 °C if kept sterile.
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.