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C5533

Sigma-Aldrich

Collagen from human placenta

Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

Synonym(s):
Collagen Type 4
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

human placenta

sterility

non-sterile

product line

BioReagent

form

powder

packaging

pkg of 5 mg

application(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

shipped in

wet ice

storage temp.

−20°C

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General description

Type IV collagens are localized to the basement membrane and they are network-forming proteins. It is encoded by six different genes: collagen type IV α1 (COL4A1), COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. Both COL4A1 and COL4A2 genes are mapped to human chromosome 13q34. The proteins encoded by the two genes share 45% homology. They contain: a non-collagenous (NC1) domain, triple-helical collagenous domain and Gly-Xaa-Yaa repeats in common. Both COL4A3 and COL4A4 genes are located on human chromosome 2q36.3. In tissues, type IV collagens are present as procollagens.

Application

Collagen from human placenta has been used:
  • To solubilize extracellular matrix for surface functionalization.
  • To induce endothelial cell and rat pluripotent cell differentiation.
  • As an adherent in cell migration/invasion assay.
  • To assess the bioelectric effects of quinine on airway epithelial cells.
  • To study the selective toxicity of engineered lentvirus lytic peptides.
  • In particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus.
  • In a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Biochem/physiol Actions

Type IV collagen maintains cell division and growth. Abnormal collagen secretion affects basement membrane formation. The collagen type IV, α1 (COL4A1) gene is overexpressed in gastric cancer. Mutation in COL4A1 contributes to intracerebral hemorrhage in adults and children. Cerebrovascular disease is resulted due to COL4A1 and COL4A2 mutations. Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis.
During development, collagen IV is ubiquitously distributed in the basement membranes (BMs). During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining the BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease, Goodpasture syndrome, is due to the pathogenic autoantibodies targeting the collagen IV α3 chain. Mutations in the COL4A5 gene are associated with Alport syndrome.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It was prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A cell migration device that maintains a defined surface with no cellular damage during wound edge generation
Doran MR, et al.
Lab on a chip, 9(16), 2364-2369 (2009)
Transcriptome characteristics and X-chromosome inactivation status in cultured rat pluripotent stem cells
Vaskova EA, et al.
Stem Cells and Development, 24(24), 2912-2924 (2015)
Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis
Banushi B, et al.
Nature Communications, 7, 12111-12111 (2016)
Identification of COL4A1 as a potential gene conferring trastuzumab resistance in gastric cancer based on bioinformatics analysis
Huang R, et al.
Molecular Medicine Reports, 17(5), 6387-6396 (2018)
Phenotype variability in a large Spanish family with Alport syndrome associated with novel mutations in COL4A3 gene
Cervera AC, et al.
BMC Nephrology, 18(1), 325-325 (2017)

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