pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
cell culture | mammalian: suitable
saline: 0.05 M (Tris-buffered)
human ... FN1(2335)
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12 - Non Combustible Liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Recommended storage of this material is 2-8 °C. Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Fibronectin 0.1% solution is a sterile product. It is tested for sterility. The sterility test results can be found on the lot-specific certificate of analysis.
This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.