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F0895

Sigma-Aldrich

Fibronectin human plasma

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

Synonym(s):
Fibronectin
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

Quality Level

biological source

human plasma

sterility

sterile

product line

BioReagent

form

liquid

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

0.1% (Solution)

application(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

saline: 0.05 M (Tris-buffered)

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.

Application

Fibronectin human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should I store and handle Product F0895, Fibronectin from human plasma?

    Recommended storage of this material is  2-8 °C.  Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

  4. Is Product F0895, Fibronectin from human plasma, sterile?

    Fibronectin 0.1% solution is a sterile product.  It is tested for sterility.  The sterility test results can be found on the lot-specific certificate of analysis.

  5. What is the solvent for Product F0895, Fibronectin from human plasma?

    This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.

  6. How much Product F0895, Fibronectin from human plasma, do I use for my application?

    Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Jonathan Iu et al.
Tissue engineering. Part A, 23(17-18), 1001-1010 (2017-05-10)
The intervertebral disc (IVD) is composed of nucleus pulposus (NP) surrounded by multilamellated annulus fibrosus (AF), and is located between the vertebral bodies. Current treatments for chronic neck or low back pain do not completely restore the functionality of degenerated...
Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.
Altomare L, et al.
The International Journal of Artificial Organs, 33(8), 535-543 (2010)
The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.
Farcas M A, et al.
BioMedical Engineering OnLine, 8(1), 30-30 (2009)
Ana Bagulho et al.
Redox biology, 6, 454-460 (2015-09-27)
The molecular processes that are crucial for cell function, such as proliferation, migration and survival, are regulated by hydrogen peroxide (H2O2). Although environmental cues, such as growth factors, regulate redox signaling, it was still unknown whether the ECM, a component...
Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
Birkenkamp-Demtroder K, et al.
British Journal of Cancer, 105(4), 552-552 (2011)

Articles

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Attachment Factors for 3-Dimensional Cell Culture

Attachment Factors for 3-Dimensional Cell Culture

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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