F0895

Fibronectin human plasma

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

• Synonym(s): Fibronectin
• MDL number: MFCD00131062
• eCl@ss: 42020141
• NACRES: NA.75

Properties

• Quality Level: 200
• biological source: human plasma
• sterility: sterile
• product line: BioReagent
• form: liquid
• packaging: pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• concentration: 0.1% (Solution)
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑5 μg/cm²
• solubility: saline: 0.05 M (Tris-buffered)
• UniProt accession no.: P02751
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... FN1(2335)

Description

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.

Application

Fibronectin human plasma has been used in the determination of H₂O₂ consumption rate. It has also been used in coating the Sylgard^® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable