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Key Documents

FC014

Sigma-Aldrich

Fibronectin, Bovine

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

biological source

bovine

Quality Level

100

assay

~95% (affinity purified, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1.0 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)

UniProt accession no.

P02751
P07589

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

Related Categories

General description

Bovine fibronectin was purified from bovine plasma over a gelatin agarose column. A single band at 220kDa (reduced) is identified, corresponding to intact fibronectin under reduced conditions.
Product Source: Bovine plasma

Application

ELISA, Ouchterlony and immunoblotting.

Physical form

Liquid in a solution of 2M Urea in 0.05 M Tris pH 7.0.

Storage and Stability

Aliquot and store up to 12 months at -20°C. For short term (1-2 weeks) use, storage at 2-8°C is acceptable. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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E Ruoslahti et al.
The Biochemical journal, 193(1), 295-299 (1981-01-01)
Human fibronectin was isolated from second-trimester amniotic fluid, from amniotic fluid obtained at term and from adult plasma. The amniotic-fluid fibronectins had a slightly higher apparent molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis than the plasma fibronectin. Early- and late-amniotic-fluid

Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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