MilliporeSigma
All Photos(1)

Documents

F4759

Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

All Photos(1)
Synonym(s):
Fibronectin
EC Number:
289-149-2
MDL number:
MFCD00131062
eCl@ss:
42020141
NACRES:
NA.75

biological source

bovine plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F2006F1141341631
Fibronectin bovine plasma powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F4759

Fibronectin bovine plasma

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F2006

Fibronectin human plasma

Fibronectin bovine plasma solution, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

F1141

Fibronectin bovine plasma

Sigma-Aldrich

Sigma-Aldrich

341631

Fibronectin, Bovine Plasma

form

powder

form

lyophilized powder

form

solution

form

liquid

mol wt

~450 kDa

mol wt

450 kDa

mol wt

-

mol wt

-

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 5 x 5 mg, pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

pkg of 1 mg, pkg of 2 mg, pkg of 5 mg

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

solubility

-

solubility

-

General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.
Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Fibronectin Adhesion-promoting Peptide ≥95% (HPLC)

Sigma-Aldrich

F3667

Fibronectin Adhesion-promoting Peptide

Fibronectin human recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Sigma-Aldrich

ECM001

Fibronectin human

Fibronectin Fragment III1-C human recombinant, expressed in E. coli, lyophilized powder

Sigma-Aldrich

F3542

Fibronectin Fragment III1-C human

Sigma-Aldrich

Sigma-Aldrich

F0556

Fibronectin solution human fibroblasts

Fibronectin Proteolytic Fragment from human plasma lyophilized powder, 45 kDa

Sigma-Aldrich

F0162

Fibronectin Proteolytic Fragment from human plasma

Roche

Roche

FIBRP-RO

Fibronectin (pure)

Mouse Decorin ELISA Kit for serum, plasma and cell culture supernatant

Sigma-Aldrich

RAB0141

Mouse Decorin ELISA Kit

Toru Yoshihara et al.
PLoS genetics, 14(8), e1007545-e1007545 (2018-08-17)
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using
R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft.
Cigdem Aydin et al.
Human gene therapy, 18(1), 39-50 (2006-12-26)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not
Wagner Shin Nishitani et al.
PloS one, 10(5), e0126440-e0126440 (2015-05-07)
A cell mechanical stimulation equipment, based on cell substrate deformation, and a more sensitive method for measuring adhesion of cells were developed. A probe, precisely positioned close to the cell, was capable of a vertical localized mechanical stimulation with a
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
View All Related Papers

Articles

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service