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F4759

Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

Synonym(s):
Fibronectin
EC Number:
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

biological source

bovine plasma

Quality Level

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

application(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

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General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.
Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Wagner Shin Nishitani et al.
PloS one, 10(5), e0126440-e0126440 (2015-05-07)
A cell mechanical stimulation equipment, based on cell substrate deformation, and a more sensitive method for measuring adhesion of cells were developed. A probe, precisely positioned close to the cell, was capable of a vertical localized mechanical stimulation with a...
Cigdem Aydin et al.
Human gene therapy, 18(1), 39-50 (2006-12-26)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not...
R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft....
Huayu Zhang et al.
International journal of molecular sciences, 21(4) (2020-02-27)
In normal physiology, endothelial cells (ECs) form a vital barrier between the blood and underlying tissue controlling leukocyte diapedesis and vascular inflammation. Emerging data suggest that neuronal guidance cues, typically expressed during development, have roles outside the nervous system in...
Birgit Ungerböck et al.
Lab on a chip, 13(8), 1593-1601 (2013-02-28)
In this work we present a high resolution oxygen imaging approach, which can be used to study 2D oxygen distribution inside microfluidic environments. The presented setup comprises a fabrication process of microfluidic chips with integrated luminescent sensing films combined with...

Articles

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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