MilliporeSigma
All Photos(1)

FC010

Sigma-Aldrich

Human Plasma Fibronectin Purified Protein

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

eCl@ss:
32160405
NACRES:
NA.75

Quality Level

biological source

human

sterility

sterile; sterile-filtered

assay

~95% (SDS-PAGE)

form

liquid

mol wt

220 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

General description

Product Source: Human donor plasma.
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

Packaging

10 mg in 10 x 1 mg
5 mg in 5 x 1 mg

Application

Immunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

Physical form

Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.

Storage and Stability

Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.

Analysis Note

A double band of 220 kDa is present under reduced conditions.hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Articles

Attachment Factors for 3-Dimensional Cell Culture

Attachment Factors for 3-Dimensional Cell Culture

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service