FC010

Human Plasma Fibronectin Purified Protein

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

• eCl@ss: 32160405
• NACRES: NA.75

Properties

• Quality Level: 300; 100
• biological source: human
• sterility: sterile; sterile-filtered
• assay: ~95% (SDS-PAGE)
• form: liquid
• mol wt: 220 kDa
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• input: sample type pancreatic stem cell(s); sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type epithelial cells; sample type hematopoietic stem cell(s); sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s)
• NCBI accession no.: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• UniProt accession no.: P02751
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... FN1(2335)

Description

General description

Product Source: Human donor plasma.

Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

Packaging

10 mg in 10 x 1 mg

5 mg in 5 x 1 mg

Application

Immunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

Physical form

Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.

Storage and Stability

Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.

Analysis Note

A double band of 220 kDa is present under reduced conditions.hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3