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11243217001

Roche

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Synonym(s):
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
CAS Number:
MDL number:

Quality Level

biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

sterility

sterile; sterile-filtered

assay

≥90% (SDS-PAGE)

form

solution

mol wt

900 kDa

packaging

pkg of 1 mg (2 ml)

application(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Lamb2(16779)

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General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Quality

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Sequence

Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Physical form

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane

Preparation Note

Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study...
Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was...
Elias Theodorou et al.
Genes & development, 23(5), 575-588 (2009-03-10)
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced...
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated...

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