All Photos(1)



Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

Bandeirea simplicifolia agglutinin, BS-I

Quality Level


FITC conjugate


lyophilized powder


<50 μg per mL agglutination activity (using human blood group B erythrocytes)


Protein, ≥70% Lowry (balance sodium citrate)

extent of labeling

≥1 mol FITC per mol lectin

storage temp.


Looking for similar products? Visit Product Comparison Guide

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.


Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection in murine astrocytes culture by staining the culture with lectin BS1-B4. It has also been used in the preparation of staining solution to incubate the tissue sections.


0.2, 1 mg in serum bottle
Package size based on protein content by Lowry.

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Product Information Sheet

Quotes and Ordering

Evgeny Levin et al.
Scientific reports, 6, 38928-38928 (2016-12-14)
Delivery and expression of recombinant genes, a key methodology for many applications in biological research, remains a challenge especially for mature neurons. Here, we report easy, highly efficient and well tolerated transduction of adult peripheral and central neuronal populations of
K A Vessey et al.
The Journal of comparative neurology, 519(3), 506-527 (2010-12-31)
Retinal neovascularization, such as that occurring in proliferative diabetic retinopathy and retinopathy of prematurity, can have serious effects on visual function. By using a mouse model of neovascularization, oxygen-induced retinopathy (OIR), the interplay among angiogenesis, neuronal function, and the macro-
Theresa Puthussery et al.
The Journal of comparative neurology, 513(4), 430-440 (2009-01-31)
We have previously demonstrated that photoreceptors express P2X(7) purinoceptors. These excitatory receptors are activated by extracellular adenosine 5'-triphosphate (ATP) and have been implicated in neurodegeneration in other parts of the central nervous system (CNS). In this study we examined whether
Reduced adipose tissue oxygenation in human obesity: evidence for rarefaction, macrophage chemotaxis, and inflammation without an angiogenic response
Pasarica M, et al.
Diabetes, 58(3), 718-725 (2009)
Magdalena Pasarica et al.
The Journal of clinical endocrinology and metabolism, 95(8), 4052-4055 (2010-05-15)
Adipose tissue in obese individuals is characterized by reduced capillary density and reduced oxygenation. Our objective was to test whether hypoxia is associated with reduced antilipolytic effect of insulin. Twenty-one lean and obese individuals participated in this cross-sectional study at

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service